Analyze both Gene Copy Numbers and Gene Expression for the Same Set of Genes
The importance of determining gene copy number or copy number variation (CNV) is increasing as it is believed to be responsible for
Phenotypical variability seen in humans during drug treatment
Disease susceptibility including HIV infection and lupus (SLE)
Complex behavioral traits including autism, schizophrenia, etc
Development of certain cancers
StellARray™ qPCR arrays and GPR&trade analysis software are designed to be used for both expression profiling as well as
the determination of gene copy number. By applying genomic DNA as template the StellARray™ System easily and accurately
determines the number of copies of a gene which is/are responsible for the expression levels observed.
Fig 1 GPR based genomic DNA copy number variation (CNV) analysis. Using the 384-well Lymphoma and Leukemia StellARray™ individual
gDNA samples (biological replicates) from five male C57BL/6J and five female C57BL/6J mice were compared for a GPRTM based genomic DNA copy
number variation (gDNA CNV) analysis. The gene content of this StellARray includes 12 genes physically located on the mouse X chromosome.
The expected CNV is two fold due to the females having XX (2 copies of X) and males having XY (one copy of X). This '5 vs. 5' example
identified all 12 X-specific genes with statistical significance, ranking them as the top 12 'hitters', and providing an average Fold Change
value of 2.0136 with a standard deviation of 0.112. Additionally, there are no other X chromosome genes within this gene set thus GPR was
highly efficient at determining 2 fold differences.
