Reagent Calculator

384 Super Mix Recipe

ul	2x SYBR Green Master Mix
ul	dH2O

For Individual Sample Working Solutions

4013 ul	Super Mix (from above)
211 ul	Template

• STEP 1: Remove foil from plate.
• STEP 2: Pipet 10ul per well
• STEP 3: Seal plate.
• STEP 4: Centrifuge plate or hand-spin reagents to well bottom. (Don't worry about floating bubbles.)
• STEP 5: Incubate at room temperature for at least 15 minutes.
• STEP 6: Run plate in Real-Time PCR instrument.
  

96 Fast Super Mix Recipe

ul	2x SYBR Green Master Mix
ul	dH2O

For Individual Sample Working Solutions

1003 ul	Super Mix (from above)
53 ul	Template

• STEP 1: Remove foil from plate.
• STEP 2: Pipet 10ul per well
• STEP 3: Seal plate.
• STEP 4: Centrifuge plate or hand-spin reagents to well bottom. (Don't worry about floating bubbles.)
• STEP 5: Incubate at room temperature for at least 15 minutes.
• STEP 6: Run plate in Real-Time PCR instrument.
  

96 Super Mix Recipe

ul	2x SYBR Green Master Mix
ul	dH2O

For Individual Sample Working Solutions

2006 ul	Super Mix (from above)
106 ul	Template

• STEP 1: Remove foil from plate.
• STEP 2: Pipet 20ul per well
• STEP 3: Seal plate.
• STEP 4: Centrifuge plate or hand-spin reagents to well bottom. (Don't worry about floating bubbles.)
• STEP 5: Incubate at room temperature for at least 15 minutes.
• STEP 6: Run plate in Real-Time PCR instrument.